Acuitas Lighthouse is the first service of its kind to use genomics and informatics to predict antibiotic resistance and it is being developed to provide real-time suggestions for treatment.  Drawing on genotype and phenotype data from 15,000 clinical isolates collected from hospitals worldwide, it predicts antibiotic resistance in specific microbes and generates simple reports that tell which drugs are likely to be effective and which ones are likely to be ineffective. Data from the Acuitas AMR Gene Panel (RUO) or from OpGen’s Acuitas Whole Genome Sequencing (RUO) service can be loaded into the Acuitas Lighthouse to produce instant antibiotic resistance prediction reports.

 

Accurate and Reliable Antibiotic Resistance Predictions

Average Sensitivity and Specificity for Genotypic Prediction of Phenotypic Resistance

OrganismAvg. SensitivityAvg. SpecificityAntibiotics
E. coli91%90%Aztreonam, Cefazolin, Cefepime, Cefotaxime, Ceftazidime, Ceftriaxone, Ciprofloxacin, Levofloxacin, Gentamicin, Tobramycin, Trimethoprim-sulfamethoxazole, Piperacillin
K. pneumoniae90%83%Aztreonam, Cefazolin, Cefepime, Cefotaxime, Ceftazidime, Ceftriaxone, Ciprofloxacin, Levofloxacin, Imipenem, Gentamicin, Tobramycin, Trimethoprim-sulfamethoxazole
P. mirabilus81%89%Ampicillin, Cefazolin, Cefotaxime, Ceftazidime, Ceftriaxone, Tobramycin, Trimethoprim-sulfamethoxazole, Piperacillin
P. aeruginosa85%82%Ciprofloxacin, Levofloxacin
Algorithm Training Set Data
7,527 isolates, geographically diverse
Certain combinations of the 4 pathogens and the antibiotics Ampicillin, Imipenem, Meropenem, Ertapenem, Aztreonam, Cefepime, Ceftazidime, Gentamicin, and Tobramycin are analyzed by a different model and only resistance is reported when specific antibiotic resistance genes are detected.  These combinations are not included in the table above.
Walker et al., Predicting Antibiotic Resistance in Gram-Negative Bacilli from Resistance Genes.  Antimicrobial Agents and Chemotherapy. 63:e02462-18

How it works

Step 1 - Genetic Results from PCR Analysis antibiotic resistance
Genetic Results from PCR Analysis
Upload to Acuitas Lighthouse
Upload to Acuitas Lighthouse
Instant Reporting of Antibiotic Resistance Prediction
Instant Reporting of Resistance Prediction
In development.
For Research Use Only. Not for use in diagnostic procedures.