Mapping Genomes in 4 Steps
The Argus® Whole Genome Mapping System and MapSolver™ analysis software provide a straightforward, automated workflow for evaluating whole genomes and chromosomes in as little as 24 hours for smaller genomes.
Using OpGen’s optimized DNA extraction kit, high molecular weight DNA needed to produce Whole Genome Maps can be extracted in as little as two hours.
2. Immobilize & Digest
Single DNA molecules are flowed through microfluidic channels and immobilized on a charged glass surface. The immobilized DNA is digested, maintaining the fragment order.
3. Measure & Assemble
The DNA fragments are stained with fluorescent dye; fragment length is proportional to fluorescence intensity. By overlapping fragment patterns, the single-molecule maps are assembled to produce a Whole Genome Map that provides a minimum 30 X coverage.
The MapSolver™ Analysis tool provides powerful features to evaluate and compare Whole Genome Maps. Discover genetic variation, perform high-resolution epidemiology, or accelerate whole-genome sequencing.
- View and compare genomes
- Identify important genetic variations
- Upload sequence contig data to align contigs against an ordered whole-genome scaffold
- Use the Whole Genome Map as an independent source to guide sequence placement, confirm assemblies, identify misassemblies and gaps, and finish de novo sequences