Be more certain when confirming or ruling out transmission events with Acuitas Whole Genome Sequence Analysis. Core Genomic Multi-locus Sequencing Typing (cgMLST), based on approximately 1000 genes or more per bacterial species, provides higher resolution strain typing than conventional MLST. Our strain typing services provide a more precise picture of isolate relationships.
Our schemas allow us to strain type isolates for more than 20 Gram-negative and Gram-positive species and provide a dendrogram that depicts strain relatedness for the isolates analyzed.
Strain typing analysis is also available for customer generated sequencing data or assembled whole genome sequences. Please contact us to discuss.
Strain Typing Workflow:
Submit bacterial isolates
Whole genome sequencing data generated
WGS assembly and strain typing
Dendrogram of strain relatedness delivered within 2 weeks.
See a sample report.
OpGen has developed customized cgMLST schema for common Gram-negative and Gram-positive bacilli: A. baumannii, C. difficile, C. freundii, E. aerogenes, E. cloacae, E. coli, E. faecalis, E. faecium, K. pneumoniae, K. oxytoca, K. variicola, P. aeruginosa, P. mirabilis, S. aureus, S. marcescens and more….
Specimen Type: Single colony on bacterial slants. Download the Client Services Guide for sample preparation and shipping instructions.
Call 301.869.9683 or email to discuss your needs for Transmission Investigation Sequencing and Analysis.