AMR Gene Panel
We help healthcare professionals, by providing a comprehensive AMR testing solution, to rapidly optimize therapy for compromised patients based on the ability to identify resistance days earlier than conventional AST.
The FDA-cleared Acuitas AMR Gene Panel detects and differentiates antibiotic resistance markers to one or more antimicrobial agents in isolated bacterial colonies of Pseudomonas aeruginosa, Enterococcus faecalis, or members of Enterobacterales.
The antimicrobial resistance gene results are reported by the Acuitas AMR Gene Panel for specific combinations of bacterial pathogens and their associated genetic resistance markers.
| Organism | Reported AMR Gene Marker |
|---|---|
| Citrobacter freundii complexa | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Citrobacter koseri | KPC, OXA-48 |
| Enterobacter cloacae complexb | CTX-M-1, CTX-M-9, KPC, TEMd |
| Enterococcus faecalis | vanA |
| Escherichia coli | AAC, ANT, CMY, CTX-M-1, CTX-M-2, CTX-M-9, DFR, gyrA Mutantc, KPC, MCR-1e, OXA-1, OXA-9, SHVd, Sul1, Sul2, TEMd |
| Klebsiella aerogenes | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella michiganensis | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella oxytoca | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella pneumoniae | AAC, AAD, APH, CMY, CTX-M-1, CTX-M- 9, DFR, DHA, IMP, KPC, NDM, OXA-1, OXA-9, OXA-48, RMT, Sul1, Sul2, TEMd |
| Klebsiella quasipneumoniae | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella variicola | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Morganella morganii | CTX-M-1, KPC, NDM, OXA-48 |
| Proteus mirabilis | AAC, ANT, APH, armA, CMY, CTX-M-1, CTX-M-2, CTX-M-9, DFR, KPC, NDM, OXA-1, OXA-9, OXA-48, Sul2, TEMd, VEB, VIM |
| Providencia rettgeri | NDM |
| Providencia stuartii | NDM |
| Pseudomonas aeruginosa | AAC, ANT, CTX-M-1, CTX-M-2, gyrA Mutantc, KPC, NDM, OXA-1, PER, SHVd, TEMd, VEB, VIM |
| Raoultella ornithinolytica | KPC, NDM, OXA-48 |
| Raoultella planticola | KPC |
| Serratia marcescens | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
- Citrobacter freundii complex = C. freundii, C. braakii, C. werkmanii and C. youngae.
- Enterobacter cloacae complex = E. asburiae, E. cloacae, E. hormaechei, E. kobei and E. ludwigii.
- PCR assays associated with fluoroquinolone resistance detect and differentiate wild type and mutant variants of gyraseA at amino acid position 87 for E. coli and position 83 for P. aeruginosa.
- PCR assays for SHV and TEM detect several sequence variants for the two genes, respectively, at amino acid positions 156 and 104 associated with wild type penicillin resistance and mutations associated with ESBL phenotypes.
- The panel includes an assay for the detection of the mobilized colistin genetic determinant MCR-1 in E. coli.
Actionable
What does this mean to clinicians?
The identification of genetic determinants associated with antibiotic nonsusceptibility or resistance can aid clinicians in the management of patients with known or suspected antibiotic non-susceptible or resistant bacterial infections.
- Results in about 2.5 hours
- Rapidly rule-in resistance
- Reassess empiric treatment
- Isolate patient prior to final AST
- Support antibiotic stewardship
Multiplexed
What makes the comprehensive Acuitas® AMR Gene Panel unique?
- FDA cleared
- 28 genetic antimicrobial resistance (AMR) markers (including MCR-1, a marker of public health importance associated with reduced inhibitory activity of polymyxins)
- 9 classes of antibiotics (detect and report AMR associated with resistance to select drugs in the following antibiotic groups: aminoglycosides, carbapenems, cephalosporins, fluoroquinolones, penicillins, polymyxins, sulfonamides, trimethoprim, and vancomycin)
- Any primary sample source (from isolated bacterial colony)
Aminoglycosides | Carbapenems | Cephalosporins | Fluoroquinolones | Penicillins | Polymyxins | Sulfonamides | Trimethoprim | Vancomycin |
|---|---|---|---|---|---|---|---|---|
| AAC | CMY | CMY | E. coli gyrA Mutant | OXA-1 | MCR-1 | Sul1 | DFR | vanA |
| AAD | DHA | DHA | P. aeruginosa gyrA Mutant | SHV | Sul2 | |||
| ANT | IMP | CTX-M-1 | TEM | |||||
| APH | KPC | CTX-M-2 | ||||||
| armA | NDM | CTX-M-9 | ||||||
| RMT | OXA-48 | OXA-9 | ||||||
| PER | VEB | |||||||
| VIM |
Revolutionary
The Acuitas AMR Gene Panel is unparalleled in delivering high quality rapid molecular AMR results.
- The largest AMR panel cleared by the FDA, covering carbapenems and much more
- Support antibiotic stewardship with the potential to guide patient care at least a day earlier than traditional phenotypic AST methods
- Can help with infection prevention and control initiatives, patient isolation procedures, and surveillance of serious or emerging AMR threats
- Accurate detection of a broad array of AMR markers (from isolated bacterial colony)
How does it work?
The comprehensive Acuitas Gene Panel can detect several AMR gene variants with demonstrated association for phenotypic antibiotic non-susceptibility in a few hours of obtaining a bacterial isolate.
Each test report indicates detection of applicable antimicrobial resistance gene variants as “Detected”, “Not Detected” or “NA/NR”.
