Advanced AMR Marker Detection & Quantification
ARESasp is a target-enrichment-based sequencing service for the comprehensive detection and quantification of more than 8,000 distinct AMR genes (>14,000 AMR markers). The ARESasp service can be applied to extracted DNA from many sources including environmental, such as natural water sources or wastewater, as well as human or veterinary samples.
Data Analysis & Interpretation
Comprehensive screening for > 14,000 AMR markers
The ARESasp is as sensitive as qPCR and as comprehensive as metagenomic sequencing, thus capturing the full AMR marker spectrum in a cost-efficient way.
Sensitive & Precise AMR profiling
ARESasp was tested using bacterial communities with high and low DNA content. In this analysis, sensitive and precise marker detection (>94%) was achieved on samples with a broad range of bacterial to non-bacterial DNA ratios.
Quantification of AMR marker copy number
ARESasp incorporates internal controls to monitor assay performance and enable absolute quantification of AMR markers. The copy number of individual markers can be compared across samples and between time points.
Accessible through the AREScloud web application
ARESasp is available as a feature in AREScloud, a user-friendly web application for interactive sequence analysis, visualization, and comparison.
Comprehensive AMR marker surveillance
Environmental, veterinary or human samples
Standardization to support inter-sample comparisons
Identification of emergent threats
Resistome profiling of simple and complex microbial samples
Sample to result service with validated SOPs
Quantification of detected AMR markers
Project management and reporting via AREScloud
Actionable AMR Marker Detection and Quantification
Hybrid capture assay for the selection of DNA fragments with AMR marker sequences
Short-read sequencing with increased sequencing depth on AMR markers
AMR marker detection using sequence similarity and detection of known SNPs
AMR marker quantification by calculating normalized sequence copy numbers for every detected marker